anti-dffb抗体供应商
catalog number : byk-0043r
quantity size : 0.1ml (1mg/ml,lyophilized or liquid.buffer=0.01m pbs,ph 7.4 with 10 mg/ml bsa and 0.1% sodium azide.reconstitute with 0.1ml sterile distilled water.)
background: apoptosis is a cell death process that removes toxic and/or useless cells during mammalian development. the apoptotic process is accompanied by shrinkage and fragmentation of the cells and nuclei and degradation of the chromosomal dna into nucleosomal units. dna fragmentation factor (dff) is a heterodimeric protein of 40-kd (dffb) and 45-kd (dffa) subunits. dffa is the substrate for caspase-3 and triggers dna fragmentation during apoptosis. dff becomes activated when dffa is cleaved by caspase-3. the cleaved fragments of dffa dissociate from dffb, the active component of dff. dffb has been found to trigger both dna fragmentation and chromatin condensation during apoptosis. alternatively spliced transcript variants encoding distinct isoforms have been found for this gene but the biological validity of these variants has not been determined.
specificity :
anti-dffb is a rabbit polyclonal antibody unconjugated specific for dffb of mouse,rat,rabbit,guinea piguse for western blotting,elisa,immunoprecipitation and immunohistochemistryprotein g affinity chromatography purification, purity :>95%isotype: iggmol wt: 40kda
application :
western blotting=1:100-500 immunohistocry 1:100-500 elisa= 1:500-1000ihc-p=1:100-500ihc-f=1:100-500if=1:100-500 ip=1:20-100klh conjugated synthetic peptide derived from mouse dff-40 beta (201-260aa)optimal working dilutions must be determined by the end user.
storage: store at -20℃ for one year. avoid repeated freeze/thaw cycles. the lyophilized antibody is stable at room temperature for at least one month and for greater than a year when kept at -20℃. when reconstituted in sterile ph 7.4 0.01m pbs or diluent of antibody, the antibody is stable for at least six weeks at 2-4 ℃
important note: this product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications.